Incorporation of Deoxyribonucleic Acid Precursors by T4 Deoxyribonucleic Acid-Protein Complexes Retained on Glass Fiber Filters

Enzymatic Synthesis of Deoxyribonucleic Acid. Deoxyribonucleic Acid By

MISSOURI The enzymatic synthesis of DNA1 in sufficient excess over the DNA used to prime the reaction21 a has enabled us to examine the chemical composition of the synthetic product. As has already been demonstrated, this DNA consists of deoxy-nucleotides Linked by typical 3'-5' phosphodiester bonds, and all four of the deoxy

Enzymatic Synthesis of Deoxyribonucleic Acid. Iii. the Incorporation of Pyrimidine and Purine Analogues into Deoxyribonucleic Acid.

Equal incorporation of both parental bacteriophage T7 deoxyribonucleic acid strands into intracellular concatemeric deoxyribonucleic acid.

After infection of Escherichia coli B with radiolabeled T7 bacteriophage, the parental deoxyribonucleic acid label was found in both polynucleotide chains of the intracellular T7 concatemer.

Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4.

DNA polymerase induced in Escherichia coli by phage T4 am N82 has been prepared in a highly purified state. The molecular weight of about 112,000 for this enzyme compares with a value of 109,000 for the host (E. coli) DNA polymerase. The amino acid compositions of the two enzymes, however, are distinctive, especially the half-cystine value of 15 residues for the phage enzyme and 3 for the bacte...

Deoxyribonucleic acid-deoxyribonucleic acid hybridization assay for replication origin deoxyribonucleic acid of Escherichia coli.

Deoxyribonucleic acid (DNA)-DNA hybridization on nitrocellulose filters can be used to assay for replication origin DNA from Escherichia coli if the DNA attached to the filters is enriched for the replication origin sequences. Such DNA can be readily isolated from very rapidly growing cells. When low amounts of this DNA were attached to filters, radioactively labeled DNA from the replication or...